Figure 3. Schematic showing breeding strategies to determine whether the occurrence of Fak floxed allele recombination is at germline level.

(a) Female FAK^F/R mice were mated with male wild type mice. The table below the scheme shows the observed genotype distribution. The numbers of animals per total number of animals (n=45) is shown in parentheses. (b) Representative PCR reaction showing genotyping result of the offspring of female FAK^F/^R mice and male wild type mice. Primer pair P1/P2 was used to amplify WT and floxed Fak allele (Flox) as 800-bp and 1000-bp bands, respectively. Primer pair P1/P3 was used to amplify a 550-bp rearranged floxed Fak allele (FAK^R).