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. Author manuscript; available in PMC: 2018 Nov 15.
Published in final edited form as: Int J Cancer. 2017 Aug 8;141(10):2062–2075. doi: 10.1002/ijc.30909

Figure 5. miR-130b activates AP-1 signaling.

Figure 5

(A) Western blot of TC71 cells expressing miR-130b or scrambled control (right) western blot of fractionated lysates (left). GAPDH and HDAC1 were used as loading controls. (B) qPCR was performed to assess the expression levels of c-Jun and AP-1 targets. Total RNA was isolated 24 hours after IPA3 (10uM) treatment. P-values were determined using Student’s t-test comparing miR-130b mimic cells treated with IPA3 (10μM) to DMSO treated cells **P<0.01, ***P<0.001. (C) Assessment of c-Jun and AP-1 targets with miR-130b knockdown in MHH cells. Expression was measured by qPCR. P-values were calculated using Student’s t-test comparing scramble control to anti-miR-130b cells. *P<0.05. (D) Western blot of phosphorylated and total c-JUN after miR-130b knockdown in lysates isolated from MHH cells 48 hours post-transfection. Tubulin was used as loading control. Loading controls are identical in 4B and 5D since western blots were reprobed with different antibodies. Scr = TC71 scrambled control ES cells, 130b = TC71 miR-130b mimic ES cells, anti-130b= anti-miR-130b oligo ES cells.