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Cells cultured on the membrane do not affect the oxygen gradient to which the cells are exposed. Tiled scans of brightfield images of the membrane (top) and fluorescent images of the PtEOPK signal (bottom) demonstrate the presence of the oxygen gradient (A) with cells and (B) without cells. Scale bar: 100 μm. (C) The surface percent oxygen level as a function of position does not vary between the cell-occupied and cell-vacant condition.
