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. Author manuscript; available in PMC: 2018 Sep 18.
Published in final edited form as: Integr Biol (Camb). 2017 Sep 18;9(9):742–750. doi: 10.1039/c7ib00099e

Figure 4.

Figure 4

HIF-1α activation in endothelial cells occurs at low oxygen levels within a linear oxygen gradient. (A–B) Panels of HIF-1α immunofluorescent staining for normoxic and hypoxic homogeneous control devices. (C–D) HIF-1α immunofluorescent staining in the oxygen gradient device of cells directly above the normoxic gas supply microchannel and hypoxic gas supply microchannel, respectively. Scale bar: 20 μm. (E) Quantification of the nuclear to cytosolic ratio as a function of the position in the oxygen gradient. * p < 0.05 as compared to homogeneous normoxia.