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. 2017 Sep 18;7:11773. doi: 10.1038/s41598-017-11779-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of oral microbiota structures in the N and T groups. (a) Rarefaction analysis of bacterial 16 S rRNA gene sequences was performed to evaluate whether further sequencing would likely detect additional taxa, indicated by a plateau. Different colors represent different samples. (b) Shannon index curves were constructed to evaluate the numbers of samples likely required to identify additional taxa, indicated by a plateau. Different colors represent different samples. (c) Box plots depict differences in bacterial diversity between the N and T groups according to the Shannon index. (d) PCA at the OTU level. (e) Partial least square discriminant score plot of oral microbiota between the N and T groups. N, clinical normal samples; T, oral cancer samples.