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. 2017 Sep 18;7:11767. doi: 10.1038/s41598-017-12227-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Kaplan-Meier survival curves of mice treated with IRE or MHIRE. Pan02 pancreatic tumors with the size of 8 to 10 mm were treated with IRE or MHIRE at day 31 indicated by arrow. IRE parameters: pulse duration 100 µs, frequency 1 Hz, pulse number 90 and applied electric fields 2000–2500 V/cm. Ctr: no treatment (n = 8 mice per treatment group); IRE: treated with IRE (n = 8 mice per treatment group); MHIRE: tumor preheated with laser at 42 °C with IRE (n = 9 mice per treatment group). ***p < 0.001 for MHIRE vs IRE or Ctr by LogRank test.