Figure 3.

MK2^ΔCD11c mice accumulate stimulatory PD-L1⁻ CD103-expressing DCs. (a) CD11b and CD103-expressing DCs within tumour-infiltrating CD45⁺ leukocytes as measured by flow cytometry. (b) Representative plots showing gating strategy of CD103⁺ and CD103⁻ DCs. Cells were pre-gated for live, single, CD45⁺ CD11c⁺ cells. Frequency within parental population is indicated. (c) Surface receptor expression on intratumoural CD103⁺ and CD103⁻ DCs. (d) Ratios of PD-L1⁺/PD-L1⁻ cells within MHC-II⁺ DCs and frequency of MHC-II⁺ PD-L1⁺ cells within the indicated parental DC population. (e) Ratios of CD80⁺/CD80⁻ cells within MHC-II⁺ DCs and frequency of MHC-II⁺ CD80⁺ cells within the indicated parental DC population. Each symbol represents one individual animal. Data are presented as mean ± SEM and pooled from two to three independent experiments (n = 5–8 mice per group each). *P < 0.05. P-values were determined using Student’s t-test.