Fig. 3.

Ecto-tagged β1 integrins rescue the adhesion defect of β1 integrin KO fibroblasts. a Adhesion of parental and reconstituted β1 integrin fl/fl and KO fibroblasts 2 h after plating on wells coated with 3 μg ml⁻¹ type I collagen. b Adhesion of parental and reconstituted β1 integrin fl/fl and KO fibroblasts 1 h after plating on wells coated with 3 μg ml⁻¹ FN. c Time course of adhesion of parental and reconstituted β1 integrin fl/fl and KO fibroblasts on wells coated with 3 μg ml⁻¹ FN. d Dose-response adhesion assay of parental and reconstituted β1 integrin fl/fl and KO fibroblasts for 1 h to wells coated with increasing concentrations of FN. In all experiments, cell adhesion was measured by the absorbance at 570 nm after Crystal Violet staining. Data is shown as mean ± SEM from three independent experiments. In a, b, statistical analysis was performed using one-way repeated measures ANOVA with Dunnett post hoc test. Each column was compared to fl/fl and *p < 0.05