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. 2017 Sep 18;7:11809. doi: 10.1038/s41598-017-12235-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Relative gene expression of PKS type I gene and NRPS gene on five alternate days across strains BPSAC77, BPSAC101, BPSAC121 and BPSAC147. The expression level calculated by the formula 2^−ΔΔCt represents the x-fold difference from the calibrator and 16S rRNA gene as endogenous control. Threshold cycle (C_T) was compared with log₁₀ relative copy number of the sample from a dilution series. Error bars indicate standard error. Sample after 12^th day of incubation shows highest expression level.