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. 2017 Sep 18;7:11780. doi: 10.1038/s41598-017-12136-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The number of differentially expressed genes in rainbow trout treated with estradiol (E2). (A) Venn diagram of common differentially expressed mRNA in E2 treated fish between 2 time points (24 hours post-injection versus 72 hours post-injection). (B) Venn diagram of common differentially expressed lncRNA in E2 treated fish between 2 time points. (C) A volcano plot of differentially expressed transcripts (lncRNAs and mRNAs) between control and E2 treated group. (D–E) Unsupervised hierarchical clustering of the expression profiles of differentially expressed mRNAs (D) and lncRNAs (E) both distinguish E2 treated and control group. Up- and down-regulated genes are indicated respectively by red and blue colors.