Table 3.
Overexpression strains | MIC50 (µM) | MIC50 ratio mutant/WT |
---|---|---|
M. tb mc26230 WT | 0.55 ± 0.023‡,† | 1.00 |
M. tb mc26230-empty vector | 0.52 ± 0.010 | 0.95 |
M. tb mc26230-Ag85A | 0.55 ± 0.014 | 1.00 |
M. tb mc26230-Ag85C | 0.54 ± 0.009 | 0.98 |
M. tb mc26230-Rv0183 | 0.44 ± 0.013 | 0.80 |
M. tb mc26230-LipH | 0.72 ± 0.020*,‡ | 1.31 |
M. tb mc26230-TesA | 0.52 ± 0.012 | 0.95 |
M. tb mc26230-HsaD | 1.20 ± 0.026*,† | 2.18 |
aExperiments were performed as described in Materials and Methods. MIC50: compound minimal concentration leading to 50% growth inhibition. Values are mean of at least two independent assays performed in triplicate (CV% < 5%). MIC50 values with a commun symbol (*,‡,†) are significantly different (p-value < 0.001; ANOVA followed by Fisher’s test).