Fig. 2.

POFUT1 is required for coronary artery formation. a Coronary arteriography using red fluorescent dye showing underdeveloped coronary arteries (ca, arrow) in a E16.5 Pofut1 ^cKO heart. (b) Co-immunostaining for PECAM1 and DLL4 on E16.5 of control and Pofut1 ^cKO heart sections. High magnification images on right showing boxed regions in the individual left ventricles with smaller main arteries (arrows) and expansion of arterial plexus in the inner myocardium (between dotted lines) in a Pofut1 ^cKO heart. c SMMHC and Isolectin-B4 (IB4) co-immunostaining showing hypoplastic major coronary arteries with thin smooth muscle layer (arrow) in a Pofut1 ^cKO heart. d E11.5 ventricular explant culture (GFP labels ventricular endocardial-derived plexus, red fluorescence labels pericytes using SM22α antibodies) showing poor pericyte attachment to the plexus in a Pofut1 ^cKO explant. e, f Representative hypoxia probe staining and quantifications show expansion of inner myocardial hypoxic zone in an E16.5 Pofut1 ^cKO heart. *p < 0.01. g, h Representative VEGFA immunostaining and quantifications show expansion of VEGFA expression in the myocardial wall of an E16.5 Pofut1 ^cKO heart. *p < 0.01. en/ep endocardium/epicardium