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. 2017 Sep 14;11:284. doi: 10.3389/fncel.2017.00284

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Copyright © 2017 Kroehne, Tsata, Marrone, Froeb, Reinhardt, Gompf, Dahl, Sterneckert and Reimer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Main steps to obtain a highly pure adult zebrafish spinal oligodendrocyte progenitor cell (OPC) population: (1) Adult zebrafish spinal cord tissue is dissected. (2) The tissue is first dissociated by enzymatic digestion and then incubated with a nuclear dye. (3) The cell suspension is FACsorted based on cytoplasmic and nuclear fluorescence. (4) The purified OPCs can then be used directly for transcriptome analysis, cell culture as monoculture to study dormant OPCs or in motor neuron/OPC co-cultures to study oligodendrocyte differentiation.