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Adult zebrafish OPCs differentiate into MBP-positive mature OLs in human MN co-cultures. (A) OPCs differentiate over time in co-culture as identified by increased branching of the seeded OPCs. (B) At 16 DIC, the majority of oligodendroglial cells expresses the mature OL/myelin sheath marker protein MBP (arrowheads) while only a few cells fail to differentiate and remain MBP-negative (arrows); scale bars represent 50 μm.
