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. 2017 Sep 14;11:63. doi: 10.3389/fncir.2017.00063

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Copyright © 2017 Datta and Oliver.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Local application of U0126 into the PPT prevents selective REM sleep deprivation-induced increases in BDNF protein expression and extracellular-signal-regulated kinase 1 and 2 (ERK1/2) phosphorylation and activation in the PPT. (A) Representative western blots of pERK1/2 and α-tubulin in the PPT of animals in the following groups: (1) vehicle control injected unrestricted REM sleep (VC + RSC); (2) vehicle control injected selective REM sleep deprived (VC + RSD); (3) 0.5 nmol U0126 injected selective REM sleep deprived (0.5 nmol U0126 + RSD); (4) 1.0 nmol U0126 injected selective REM sleep deprived (1.0 nmol U0126 + RSD); and (5) 2.0 nmol U0126 injected selective REM sleep deprived (2.0 nmol U0126 + RSD). (B) Representative western blots of pMBP in the PPT of: (1) VC + RSC; (2) VC + RSD; (3) 0.5 nmol U0126 + RSD; (4) 1.0 nmol U0126 + RSD; and (5) 2.0 nmol U0126 + RSD. (C) Bars represent levels (mean ± SE) of phosphorylated ERK1/2 in the PPT of: (1) VC + RSC; (2) VC + RSD; (3) 0.5 nmol U0126 + RSD; (4) 1.0 nmol U0126 + RSD; and (5) 2.0 nmol U0126 + RSD. All analyses of pERK1/2 expression are normalized against α-tubulin. (D) Bars represent densitometric measurements (mean ± SE) from western blots of phosphorylated myelin basic protein (pMBP) levels in the PPT of: (1) VC + RSC; (2) VC + RSD; (3) 0.5 nmol U0126 + RSD; (4) 1.0 nmol U0126 + RSD; and (5) 2.0 nmol U0126 + RSD. (E) Bars represent amounts (mean ± SE) of BDNF in the PPT of: (1) VC + RSC; (2) VC + RSD; (3) 0.5 nmol U0126 + RSD; (4) 1.0 nmol U0126 + RSD; and (5) 2.0 nmol U0126 + RSD. (F) Key. Post hoc test (Bonferroni’s multiple comparisons test): triangle represents the comparison with vehicle control + REM sleep control (VC + RSC) and asterisk represents the comparison with vehicle control + selective REM sleep deprivation (VC + RSD). ∆∆∆ or ***p < 0.001.