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. 2017 Sep 14;8:1560. doi: 10.3389/fpls.2017.01560

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Copyright © 2017 Kobylińska, Reiter and Posmyk.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Micrographs of living and dying BY-2 nuclei (a–d) and whole cells (e–h) detected by AO/EB staining. Green nuclei of living cells (a,b,d,e,f,h), yellow nuclei (c,g) of PCD-dying cells. C, BY-2 cells cultured on LS medium – the control variant; MEL, BY-2 cells cultured on LS medium with 200 nM melatonin added from the beginning of culture; Pb, BY-2 cells cultured on LS medium with 15 μM Pb²⁺ added on the 4th day of culture and MEL + Pb, BY-2 cells cultured on LS medium with melatonin added from the start of the culture and with Pb²⁺ added on the 4th day of culture. Micrographs were done 4 h after lead administration.