Figure 3. Effect of rat LWBQ-medicated serums on CSE + LPS-induced release of inflammatory cytokines, cell viability, cell apoptosis, and the protein levels of MMP-9 and TIMP1 in AMs.

(A) The levels of TNF-α and IL-6 in the culture medium of AMs. (B) The cell viability was measured by MTT method. (C) The percentage of apoptotic cells was determined by flow cytometry (FCM). (D) The protein expressions of MMP-9 and TIMP1 in the AMs. (E) The mRNA expressions of MMP-9 and TIMP1 in the AMs. Abbreviations: MC, model control (CSE + LPS). **P<0.01 and ***P<0.001 compared with NC group, ^#P<0.05, ^##P<0.01, and ^###P<0.001 compared with MC group.