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. 2017 Sep 19;37(5):BSR20170798. doi: 10.1042/BSR20170798

Figure 2. miR-494 protected against liver I/R injury in SD rats.

Figure 2

SD rats (n=6 rats per group) were sham operated or subjected to 60-min liver ischemia followed by 6-h reperfusion. HIRI rats were treated with agomir-miR-494 or agomir-NC. (A) Relative miR-494 level in liver samples from difference groups was measured by qRT-PCR. miR-494 expression was normalized to U6. (B,C) Serum level of ALT, AST, LDH, and GLDH was determined using commercial kits. (D) Concentration of liver oxidative parameters MDA, TOA, and OSI. (E) TUNEL staining of hepatocellular apoptosis. (F) IHC detection of cleaved caspase-3 in different groups. (G) Hepatic expression of apoptosis-related proteins cleavage PARP, cleavage Caspase-3, Bax were determined by Western blot using β-actin as an internal control. (H) DNA fragmentation assay was performed. All experiments were performed in triplicate, and data were represented as mean ± S.D. *, P<0.05 and **, P<0.01 compared with sham surgery group; ##, P<0.01 compared with agomir-NC group.