Table 1.

Bacterial strains and plasmids in this study.

Bacterial strain	Genotype/phenotype	References or source
Pseudomonas sp. strain WBC-3	PNP and methyl parathion utilizer, wild type	Chen et al., 2002
WBC3-ΔpnpM	WBC-3 mutant with pnpM gene disrupted	This study
WBC3-ΔpnpMC	WBC3-ΔpnpM with orf 6 replaced by pnpM	This study
Pseudomonas putida PaW340	Mxy− Mtol− PNP− Strr Trp−	Williams and Murray, 1974; Liu et al., 2005b
E. coli STRAINS
DH5α	λ−Φ80dlacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 (${\text{r}}_{\text{k}}^{-}$ ${\text{m}}_{\text{k}}^{+}$) supE44 thi-1 gyrA relA1	Woodcock et al., 1989
BL21	F−ompT hsdS(${\text{r}}_{\text{B}}^{-}$${\text{m}}_{\text{B}}^{+}$) gal dcm(DE3)	Jeong et al., 2009
WM3064	thrB1 004 pro thi rpsL hsdS lacZΔM15 RP4-1360Δ (araBAD)567 ΔdapA1341::[erm pir(wt)]	Saltikov and Newman, 2003
PLASMIDS
pMD18-T	Ampr, lacZ; cloning vector	Takara
pEX18Tc	Tcr sacB+, gene replacement vector	Hoang et al., 1998
pEX18Gm	Gmr; oriT+sacB+, gene replacement vector with MCS from pUC18	Hoang et al., 1998
pTnMod-OKm	Kmr, source of neomycin phosphotransferase II gene (nptII)	Dennis and Zylstra, 1998
pEX18Tc-kanpnpM	pEX18Tc with a 1.0 kb PCR fragment carrying the flanking regions of pnpM disrupted by nptII	This study
pKOorf6-GmpnpR	pEX18Tc with a 1.0 kb PCR fragment carrying the flanking regions of orf6 disrupted by aacC1 and pnpR	Zhang et al., 2015
pKOorf6-gmpnpM	pEX18Tc with a 1.0 kb PCR fragment carrying the flanking regions of orf6 disrupted by aacC1 and pnpM	This study
pEX18Tc-cmgfp	Tcr, pEX18Tc with cm gene and gfp gene	Hu et al., 2014
pCMgfp-lacZ	pCMgfp containing lacZ	Zhang et al., 2015
pCMgfp-spClacZ	spC-lacZ translational fusion in pCMgfp carrying the sequence between positions −94 and +25	Zhang et al., 2015
pCMgfp-lpClacZ	lpC-lacZ translational fusion in pCMgfp carrying the sequence between positions −255 and +25	Zhang et al., 2015
pCMgfp-spCmlacZ	pCMgfp-spClacZ with GTT of RBS to AAA	Zhang et al., 2015
pET30a(+)	Expression vector, Kmr, C/N-terminal His•Tag/thrombin/T7•Tag, T7 lac promoter, T7 transcription start, f1 origin, lacI	Novagen
pET30a-pnpM	pET30a containing a 1 kb fragment pnpM with C-terminal His•Tag	This study
pBBR1mcs-2	Kmr, broad host range, lacPOZ′	Kovach et al., 1995
pVLT33	Kmr, RSF 1010-laclq/Ptac hybrid broad-host-range expression vector, MCS of pUCl8	de Lorenzo et al., 1993
pBBR1-tacpnpR	pBBR1mcs-2 containing a 1 kb fragment pnpR (his-tag sequence at its 3′ end) and promoter tac	Zhang et al., 2015
pBBR1-tacexpnpM	pBBR1mcs-2 containing a 1 kb fragment pnpM (his-tag sequence at its 3′ end) and promoter tac	This study
pBBR1-tacpnpMpnpR	pBBR1mcs-2 containing a 1 kb fragment pnpM, a 1 kb fragment pnpR and promoter tac	This study