Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Sep 14;8:1157. doi: 10.3389/fimmu.2017.01157

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017 Vergani, Korsunsky, Mazzarello, Ferrer, Chiorazzi and Bagnara.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Identification of chronic lymphocytic leukemia (CLL) spike-in upon dilution. (A) CLL spike-in was performed as in Figure 4A. The ds-cDNA obtained from a CLL spike-in with 1 cell per CLL was diluted at different levels (1:1, 1:2, 1:4, 1:8, and 1:16) to simulate genetic material dilution before PCR amplification step. (B) Impact of the dilution of genetic material on the identification of specific CLL IGHV genes. Every fraction of the three different replicates was sequenced. (C) The UMIG count of CLL spike-in correlates with the detection frequency after 1:16 dilution.