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. 2017 Aug 9;6(9):2052–2062. doi: 10.1002/cam4.1143

Figure 2.

Figure 2

HPV16 E6‐mediated PD‐L1 expression may be responsible for colony formation and soft agar growth ability. (A) TL‐1 and SiHa cells were transfected with E6 shRNA or E7 shRNA; TL‐4 and C33A cells were transfected with an E6‐expressing plasmid for 48 h. Western blotting analysis was performed to evaluate the expression of E6, E7, and PD‐L1 in TL‐1 and TL‐4 cells. (B) TL‐1 and SiHa cells were transfected with E6 shRNA‐ and PD‐L1‐expressing plasmid; TL‐4 and C33A cells were transfected with E6‐expressing plasmid and PD‐L1 shRNA for 48 h. The colony formation ability was evaluated after 7 days. The soft agar growth ability was determined after 14 days.