Figure 3.
The combination of Lm‐LLO‐E6 vaccine + anti‐PD‐L1 mAb resulted in stronger suppression of subcutaneous tumor growth in nude mice. The nude mice were subcutaneously injected with HPV16‐infected TL‐1 cells that expressed E6 and E7 oncoprotein. After 7 days, the mice were treated with anti‐PD‐L1 mAb (25 μg/mouse), Lm‐LLO‐E6 (E6) vaccine (5 × 106 CFU/mouse), Lm‐LLO‐E7 (E7) vaccine (5 × 106 CFU/mouse) or a combination of antibody plus one or both vaccines by peritoneal injection. The representative tumor burdens in the seven groups are illustrated. The tumor volume in the nude mice of each group was measured at 7‐day intervals from Day 0 to Day 70. Mean ± SEM values (cm3) were calculated from the tumor volumes of five nude mice in each group.