Figure 4.

Binding of BON nuclear proteins to the active Notch1 promoter region by EMSA. Biotinylated double‐stranded oligonucleotides covering the promoter region −80/−52 (A) and −75/−56 (B) were incubated with BON nuclear extracts (lane A2–5; lane B2, 3, 5, 6). Biotinylated oligonucleotide without nuclear extracts was included for control (lanes A1, B1, and B4). (C) DNA sequences of −80/−52 wild type, −80/−52 mutant, and −75/−56 oligonucleotides are shown. Competing unlabeled oligonucleotide was used in lane A4 (−80/−52 wild type) and lane A5 (−80/−52 mutant). Lane B6 included 1 μL of mouse polyclonal anti‐c‐Jun antibody. The biotinylated oligonucleotide was detected using chemiluminescent EMSA kit. NE, nuclear extract, WT, wild type, MT, mutant.