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Changes in population of particles smaller than 400nm for liposomal formulation with different charges (n = 3) for samples taken at different times (resembling different depth in the burette). The zeta potential of initial (freshly prepared liposomes) are -50, -1.4 and +56 mV for negative, neutral and positive formulation respectively and their particle size range is 30-1600nm (negative liposomes), 30-2000 (neutral liposomes) and 30-1600 (positive liposomes
