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Changes in phospholipid concentration in liposomal formulation with different charges under gravity over time (n = 3). The zeta potential of initial (freshly prepared liposomes) are -50, -1.4 and +56 mV for negative, neutral and positive formulation respectively and their particle size range is 30-1600nm (negative liposomes), 30-2000 (neutral liposomes) and 30-1600 (positive liposomes
