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. Author manuscript; available in PMC: 2018 Aug 8.
Published in final edited form as: Biochemistry. 2017 Jul 26;56(31):4039–4043. doi: 10.1021/acs.biochem.7b00456

Table 1.

Antibacterial and hemolytic effects of TetraF2W-RR (WT) and its single residue variantsa

Name Peptide Sequence S. aureus (μM) E. coli (μM) HL50 (μM) tR (min)
WT WWWLRRIW 3.1 6.2 35 12.10
W1R RWWLRRIW 12.5 25 >200 11.48
W2R WRWLRRIW 6.2 25 100 10.86
W3R WWRLRRIW 12.5 25 >200 10.77
L4R WWWRRRIW 12.5 25 >200 10.78
I7R WWWLRRRW 3.1 6.2 150 11.07
W8R WWWLRRIR 12.5 25 >200 10.87
a

C-terminal amidation.

The abbreviations used are SA, S. aureus USA300; EC, E. coli ATCC 25922; WT, wild type; W1R, residue W1 is replaced by an arginine, and so on; tR, the retention time of a peptide measured on a Waters HPLC system (for details, see the Supporting material).