Fig. 4.

M2-seq recovers helices across diverse RNA folds. Each panel shows crystallographic secondary structures and Z-score–transformed maps (square graphs) with colored labels (on both display items) marking helices and multihelix domains automatically identified by M2-net analysis. Differences in edge base pairs are not shown. Data sets are as follows: (A) P4–P6 domain of Tetrahymena ribozyme (background mutations), (B) GIR1 lariat-capping ribozyme (RNA-puzzle 5; error-prone PCR), (C) ribonuclease P catalytic domain (background mutations), and (D) adenosylcobalamin riboswitch aptamer (RNA-puzzle 6; error-prone PCR). Data for three additional RNAs of smaller length are given in Fig. S9. Table S3 compiles modeled structures.