Fig. 5.

M2-seq detects P4–P6 RNA base pairs in Xenopus egg extract. (A) Mutations across the P4–P6 transcript consistent with adenosine-to-inosine edits after exposure to undiluted Xenopus egg extract for 30 min (no DMS treatment); difference data with RNA incubated in vitro are shown. M2-seq data for P4–P6 RNA from templates prepared by error-prone PCR shown as (B) M2-seq map and then (C) transformed into Z-scores. Helix signatures automatically detected by M2-net are marked with colored labels.