Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
M2-seq signal and mutation spectra for additional experimental conditions. (A–D) M2-seq signal for DMS-modified RNA (A) transcribed from DNA template prepared from plasmid, (B) transcribed from DNA template prepared with error-prone PCR, (C and D) transcribed from PCR-assembled DNA template and PAGE-purified with UV-shadowing at ≥20 cm (C) and at ≤5 cm (D) (SI Methods). (E) Mutation spectra for RNA from indicated preparation with either no modification or DMS modification.
