Fig. 4.
Effects of the mutations of K100 and E119 located at cd1NiR binding site and the mutation of V206 in the NO transfer channel in cNOR. (A) Visible absorption spectra of the K100E, E119R, and V206W variants and wild-type cNOR in the ferric state. (B) NO consumption activity of wild type and the variants of cNOR. Enzymatic NO consumption was evaluated using a Clark-type electrode equipped with an ISO-NO mark II system (WPI). The details of the experimental condition are given in Methods. (C) Bacterial growth under anaerobic denitrification conditions. Bacterial growth was monitored at OD600 nm during anaerobic static culture in the presence of 100 mM NaNO3 at 30 °C. Error bars indicate the SD calculated from four independent experiments.