Table S2.

Truncated PTEN alpha exon 1 variants

Construct name	Long noncoding RNA genetic sequence
PTENpg1 antisense alpha exon 1 (described in ref. 3)	5′GGAAGAGGCTGCACAGTTAGAAAAGACGAAGAAGAAGCGAGAAACGCCGCCGCTGCCGGCGCCGCCCCCGCGGATGCTCACGGGTTGCTGAGAGGGGCTTCAGGCCGGGCCGGGCCGGGCCGCCGCCGCCGCCGCCGTCTCTGTCTCTCATCTCCCTCGCCTGAGCCCGGCCTCGCCTCACAGCGGCTCAACATTCAAACTTCCATCACGGCTGCAGCTTCTGAGAGGAGAGCATCTTCTAG3′
F2R1 PCR set 2 (variant 1 PTENpg1asRNA alpha, 220 bp fragment)	5′CCGGCGCCGCCCCCGCGGATGCTCACGGGTTGCTGAGAGGGGCTTCAGGCCGGGCCGGGCCGGGCCGCCGCCGCCGCCGCCGTCTCTGTCTCTCATCTCCCTCGCCTGAGCCCGGCCTCGCCTCACAGCGGCTCAACATTCAAACTTCCATCACGGCTGCAGCTTCTGAGAGGAGAGCATCTTCTAGTTTTT3′
F3R1 PCR set 3 (variant 2 PTENpg1asRNA alpha, 164 bp fragment)	5′GGGCCGGGCCGCCGCCGCCGCCGCCGTCTCTGTCTCTCATCTCCCTCGCCTGAGCCCGGCCTCGCCTCACAGCGGCTCAACATTCAAACTTCCATCACGGCTGCAGCTTCTGAGAGGAGAGCATCTTCTAGTTTTT3′
F4R1 PCR set 4 (variant 2 PTENpg1asRNA alpha, 107 bp fragment)	5′GGGTAGTGAAGGCTAGCGGGCCTCGCCTCACAGCGGCTCAACATTCAAACTTCCATCACGGCTGCAGCTTCTGAGAGGAGAGCATCTTCTAGTTTTTGGTACCTATCTGA3′
F5R2 PCR set 4 (variant 2 PTENpg1asRNA alpha, 217 bp fragment)	5′TAGTGAAGGCTAGCGCGCCGCCGCTGCCGGCGCCGCCCCCGCGGATGCTCACGGGTTGCTGAGAGGGGCTTCAGGCCGGGCCGGGCCGGGCCGCCGCCGCCGCCGCCGTCTCTGTCTCTCATCTCCCTCGCCTGAGCCCGGCCTCGCCTCACAGCGGCTCAACTCTCAAACTTCCATCATGGCTGCAGCTTCCGAGAGGTTTTTGGTACCTATCTGA3′

The experimentally determined 3a binding bulge is underlined in the F4R1 and full-length PTENpg1 alpha exon 1 and the AT insertion in bold that disrupts the 3a binding bulge for the F5R2 variant. Underlined segments represent predicted loop promoter-associated RNA-binding loci.