Fig. 5.

LF1 regulates the meristem maintenance gene OSH1. (A) qRT-PCR analysis of OSH1 in WT and lf1 panicles. (B) In situ hybridization of OsH1 in WT (B1–B3) and lf1 (B4–B6) panicles. Black arrows indicate gene expression in the lateral FM of lf1 panicles. fm, floral meristem. (C) Distribution of the conserved binding site ATGAT in the promoter regions of OsH1. Underlining indicates the DNA fragments amplified in the ChIP assays. (D and E) ChIP enrichment compared with the input sample was tested by qPCR. (D) Chromatin isolated from fresh young WT panicles and immunoprecipitated with anti-LF1 antibody. (E) Chromatin isolated from fresh young panicles of mLF1CDS-GFP transgenic plants and immunoprecipitated with anti-GFP antibody. Error bars indicate the SD of three biological repeats. (Scale bars, 50 µm.)