LF1 encodes a HD-ZIP III protein. (A) Phylogenetic tree of the LF1 protein. The phylogenetic tree was constructed using the maximum likelihood method based on the Jones–Taylor–Thornton matrix-based model. Bootstrap values were calculated from 500 replicates and are given at the branch nodes. At, Arabidopsis; Bd, Brachypodium distachyon; Os, rice; Pt, poplar; Sb, Sorghum bicolor; Ze, Zinnia elegans; Zm, maize. (B) Analysis of the subcellular localization of the LF1 protein in rice protoplasts. (Scale bars, 50 µm.) (C) Analysis of the transcriptional activation of LF1. Transcriptional activation of LF1 was tested by yeast two-hybrid analysis. The positive control was pGBKT7-53+pGADT7-T, and the negative control was pGBKT7-Lam+pGADT7-T.