Mapping |
ZIn-23F |
CCCAGGACAGAGTAGGCACAAT |
Indel |
ZIn-23R |
CGCTGTGCCTATGGAAACATACT |
Indel |
ZIn29F |
CATTACCAACCTCTACGCGC |
Indel |
ZIn29R |
CGTACTCACGGTTGTAATACTCTCC |
Indel |
ZIn27F |
GCAGCTAGTGCAAGACGGAG |
Indel |
ZIn27R |
GATGAGCCGTATGATCCGC |
Indel |
ZIn67F |
CATGTTCCAAGTATTCCTGGG |
Indel |
ZIn67R |
AGCTAGGGCACTAGCATTCG |
Indel |
RM14281F |
CATGCATGCAACTCTGCTAAACG |
SSR |
RM14281R |
ATCAGGATCCAGGAAATCGAACC |
SSR |
SNP-20F |
CCTCTTTCTATATCATGACTCGATC |
SNP |
SNP-20R |
CATGGAAGGTGGGAGGACAG |
SNP |
S-16F |
GCTCTCTCTCTTGTTCCCCTCTCA |
SSR |
S-16R |
CATGGATCGGCGATGGGT |
SSR |
RM14301F |
TCATCATCATCAGTCCAGCATCG |
SSR |
RM14301R |
GCGGCGATTGAATTGTTTCTTAGG |
SSR |
Overexpression |
LF1OE-F |
GCCGGTACCATGGCTGCGGCAGTGGCAAT |
KpnI |
LF1OE-R |
GCCACTAGTCACAAAGGACCAGTTGACGAAACAGAAG |
SpeI |
GFP-F |
GCCACTAGTATGGTGAGCAAGGGCGAGGAG |
SpeI |
GFP-R |
GCCGAGCTCTTACTTGTACAGCTCGTCCATGCCGT |
SacI |
RNA interference |
LF1Ri-F1 |
GCCGGATCCGTGATCCATGGCCGTGGCT |
BamHI |
|
LF1Ri-R1 |
GCCGGTACCGAGCCAAGCAAGCACAACCACT |
KpnI |
|
LF1Ri-F2 |
GCCGAGCTCGTGATCCATGGCCGTGGCT |
SacI |
|
LF1Ri-R2 |
GCCACTAGTGAGCCAAGCAAGCACAACCACT |
SpeI |
|
Subcellular localization |
GFP-PA7-F |
TCACCATTTACGAACGATACTCGAGATGGTGAGCAAGGGCGAGGA |
Homologous recombination |
GFP-LF1-R |
ATTGCCACTGCCGCAGCCATCTTGTACAGCTCGTCCATGCCGT |
LF1-GFP-F |
ACGGCATGGACGAGCTGTACAAGATGGCTGCGGCAGTGGCAAT |
LF1-PA7-R |
TGATGCATTCCCGGGTCTAGATCACACAAAGGACCAGTTGACGAAAC |
qRT-PCR |
Actin-F |
TGCTATGTACGTCGCCATCCAG |
|
Actin-R |
AATGAGTAACCACGCTCCGTCA |
|
qLF1-F |
CTGTGAATCAAATGTGACTACCCCT |
|
qLF1-R |
GGACGATCTTTCAATATCTCTACCAC |
|
qOsMADS1-F |
GCTGCAACTACAACTCACAGG |
|
qOsMADS1-R |
TGATGGTGAGCATGAGGGTG |
|
qOsMADS4-F |
GATGGACCACTGGAGGATGC |
|
qOsMADS4-R |
CTGGAGGTTGGGGTGGCT |
|
qOsMADS16-F |
CCGCTACCAGCAAGCCAT |
|
qOsMADS16-R |
CTTGTAGGTTTCAGTCTGTGTGG |
|
qOsMADS3-F |
AGAGAACAGGCTGGAGAAAGGC |
|
qOsMADS3-R |
GGCTGCTGTCCCCTCTCATTC |
|
qOsMADS58-F |
AACAGTGACACCTCCAACGC |
|
qOsMADS58-R |
TTGGTGATCTGTTGCTTCAGC |
|
qOsMADS13-F |
TGGCGATAATGTGAGCAACCT |
|
qOsMADS13-R |
TCCATGTTGTCGTTCTGAAGC |
|
qDL-F |
CCCATCTGCTTACAACCGCTT |
|
qDL-R |
GTTGGAGGTGGAAACCGTCG |
|
qOSH1-F |
CAGGACCTGGAGCTTCGCCAG |
|
qOSH1-R |
GGAGAGCGTGTTGAGCTGCGTCT |
|
qOsMADS6-F |
AGAGAAAGACGCAACTGATGATGG |
|
qOsMADS6-R |
AGGCTTGCTGCATGGCTCTG |
|
qOsMADS15-F |
GTCACAAACACCTCATGGGAGAGGAT |
|
qOsMADS15-R |
CACATTCTTCTGCCTCTCCACCAGT |
|
In situ |
LF1-F |
CGGATTCGGTTGGTATTGTGGC |
|
LF1SP6-R |
AGATTTAGGTGACACTATAGAACGTTAAAGCCTCTGCTCAGCCTT |
|
M16-F |
TACGGCGGCAACCACGA |
|
M16SP6-R |
AGATTTAGGTGACACTATAGAATCCAAGCACAGTTGCACACC |
|
DL-F |
ATGGATCTCGTGTCGCCGT |
|
DLSP6-R |
AGATTTAGGTGACACTATAGAATCACAACGAAGGGTGCTCT |
|
OsH1-F |
ATGGAGGAGATCTCCCACCACTT |
|
OsH1SP6-R |
AGATTTAGGTGACACTATAGAATCAGAAGAGCCGGAGGAAAGGAT |
|
M6-F |
GACGCAACTGATGATGGAACAAG |
|
M6SP6-R |
AGATTTAGGTGACACTATAGAAGACGCAGAAGGTGCAAACAGC |
|
OsMADS15-F |
TGAAGCGGATAGAGAACAAGATCAACAG |
|
OsMADS15SP6-R |
AGATTTAGGTGACACTATAGAACACATTCTTCTGCCTCTCCACCAGT |
|
Transactivation activity assay |
LF1-pGBKT7-F |
GCCGAATTCATGGCTGCGGCAGTGGCAAT |
EcoRI |
LF1-pGBKT7-R |
GCCGTCGACCACAAAGGACCAGTTGACGAAACAGAAG |
SalI |
ChIP-qPCR |
OsH1-aF |
GTGATTTATGATATATGATTTGGAGATGATT |
|
OsH1-aR |
GATTTCACTCCATTCCCAAATCAT |
|
OsH1-bF |
CCTTGACATACAACCAATGATACCAT |
|
OsH1-bR |
TATTAGTTCAGGCCATCACCCGT |
|
OsH1-cF |
CATCGTCTAAAGTACACGCAAGATTC |
|
OsH1-cR |
GAGAAACACGATACGCTTTACTACTACT |
|
OsH1-dF |
CTCTAATGATAGCTTGGAGCAAGTTC |
|
OsH1-dR |
AGTGTGTATGATAGATGGGACCAGAT |
|