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. 2017 Aug 28;114(37):9984–9989. doi: 10.1073/pnas.1700504114

Table S1.

Primers used in the study

Purpose Primer name Sequence Remarks
Mapping ZIn-23F CCCAGGACAGAGTAGGCACAAT Indel
ZIn-23R CGCTGTGCCTATGGAAACATACT Indel
ZIn29F CATTACCAACCTCTACGCGC Indel
ZIn29R CGTACTCACGGTTGTAATACTCTCC Indel
ZIn27F GCAGCTAGTGCAAGACGGAG Indel
ZIn27R GATGAGCCGTATGATCCGC Indel
ZIn67F CATGTTCCAAGTATTCCTGGG Indel
ZIn67R AGCTAGGGCACTAGCATTCG Indel
RM14281F CATGCATGCAACTCTGCTAAACG SSR
RM14281R ATCAGGATCCAGGAAATCGAACC SSR
SNP-20F CCTCTTTCTATATCATGACTCGATC SNP
SNP-20R CATGGAAGGTGGGAGGACAG SNP
S-16F GCTCTCTCTCTTGTTCCCCTCTCA SSR
S-16R CATGGATCGGCGATGGGT SSR
RM14301F TCATCATCATCAGTCCAGCATCG SSR
RM14301R GCGGCGATTGAATTGTTTCTTAGG SSR
Overexpression LF1OE-F GCCGGTACCATGGCTGCGGCAGTGGCAAT KpnI
LF1OE-R GCCACTAGTCACAAAGGACCAGTTGACGAAACAGAAG SpeI
GFP-F GCCACTAGTATGGTGAGCAAGGGCGAGGAG SpeI
GFP-R GCCGAGCTCTTACTTGTACAGCTCGTCCATGCCGT SacI
RNA interference LF1Ri-F1 GCCGGATCCGTGATCCATGGCCGTGGCT BamHI
LF1Ri-R1 GCCGGTACCGAGCCAAGCAAGCACAACCACT KpnI
LF1Ri-F2 GCCGAGCTCGTGATCCATGGCCGTGGCT SacI
LF1Ri-R2 GCCACTAGTGAGCCAAGCAAGCACAACCACT SpeI
Subcellular localization GFP-PA7-F TCACCATTTACGAACGATACTCGAGATGGTGAGCAAGGGCGAGGA Homologous recombination
GFP-LF1-R ATTGCCACTGCCGCAGCCATCTTGTACAGCTCGTCCATGCCGT
LF1-GFP-F ACGGCATGGACGAGCTGTACAAGATGGCTGCGGCAGTGGCAAT
LF1-PA7-R TGATGCATTCCCGGGTCTAGATCACACAAAGGACCAGTTGACGAAAC
qRT-PCR Actin-F TGCTATGTACGTCGCCATCCAG
Actin-R AATGAGTAACCACGCTCCGTCA
qLF1-F CTGTGAATCAAATGTGACTACCCCT
qLF1-R GGACGATCTTTCAATATCTCTACCAC
qOsMADS1-F GCTGCAACTACAACTCACAGG
qOsMADS1-R TGATGGTGAGCATGAGGGTG
qOsMADS4-F GATGGACCACTGGAGGATGC
qOsMADS4-R CTGGAGGTTGGGGTGGCT
qOsMADS16-F CCGCTACCAGCAAGCCAT
qOsMADS16-R CTTGTAGGTTTCAGTCTGTGTGG
qOsMADS3-F AGAGAACAGGCTGGAGAAAGGC
qOsMADS3-R GGCTGCTGTCCCCTCTCATTC
qOsMADS58-F AACAGTGACACCTCCAACGC
qOsMADS58-R TTGGTGATCTGTTGCTTCAGC
qOsMADS13-F TGGCGATAATGTGAGCAACCT
qOsMADS13-R TCCATGTTGTCGTTCTGAAGC
qDL-F CCCATCTGCTTACAACCGCTT
qDL-R GTTGGAGGTGGAAACCGTCG
qOSH1-F CAGGACCTGGAGCTTCGCCAG
qOSH1-R GGAGAGCGTGTTGAGCTGCGTCT
qOsMADS6-F AGAGAAAGACGCAACTGATGATGG
qOsMADS6-R AGGCTTGCTGCATGGCTCTG
qOsMADS15-F GTCACAAACACCTCATGGGAGAGGAT
qOsMADS15-R CACATTCTTCTGCCTCTCCACCAGT
In situ LF1-F CGGATTCGGTTGGTATTGTGGC
LF1SP6-R AGATTTAGGTGACACTATAGAACGTTAAAGCCTCTGCTCAGCCTT
M16-F TACGGCGGCAACCACGA
M16SP6-R AGATTTAGGTGACACTATAGAATCCAAGCACAGTTGCACACC
DL-F ATGGATCTCGTGTCGCCGT
DLSP6-R AGATTTAGGTGACACTATAGAATCACAACGAAGGGTGCTCT
OsH1-F ATGGAGGAGATCTCCCACCACTT
OsH1SP6-R AGATTTAGGTGACACTATAGAATCAGAAGAGCCGGAGGAAAGGAT
M6-F GACGCAACTGATGATGGAACAAG
M6SP6-R AGATTTAGGTGACACTATAGAAGACGCAGAAGGTGCAAACAGC
OsMADS15-F TGAAGCGGATAGAGAACAAGATCAACAG
OsMADS15SP6-R AGATTTAGGTGACACTATAGAACACATTCTTCTGCCTCTCCACCAGT
Transactivation activity assay LF1-pGBKT7-F GCCGAATTCATGGCTGCGGCAGTGGCAAT EcoRI
LF1-pGBKT7-R GCCGTCGACCACAAAGGACCAGTTGACGAAACAGAAG SalI
ChIP-qPCR OsH1-aF GTGATTTATGATATATGATTTGGAGATGATT
OsH1-aR GATTTCACTCCATTCCCAAATCAT
OsH1-bF CCTTGACATACAACCAATGATACCAT
OsH1-bR TATTAGTTCAGGCCATCACCCGT
OsH1-cF CATCGTCTAAAGTACACGCAAGATTC
OsH1-cR GAGAAACACGATACGCTTTACTACTACT
OsH1-dF CTCTAATGATAGCTTGGAGCAAGTTC
OsH1-dR AGTGTGTATGATAGATGGGACCAGAT