Fig. S5.

Functional assays with the stk-21^TAA and NCU10184^TAA alleles. (A, Upper) Asci and ascospores from the crosses between the mat A Δstk-21 mutant and a compatible (mat a) wild type (WT), Δstk-21 mutant, or Δstk-21/stk-21^TAA transformant and the Δstk-21/stk-21^TAA homozygous cross. (A, Lower) Ascospores after being incubated in YEPD for 12 h without preheating at 65 °C. (Scale bar: 20 μm.) (B) Asci and ascospores from the crosses between the mat A ΔNCU10184 mutant with a compatible (mat a) WT, ΔNCU10184 mutant, and ΔNCU10184/NCU10184^TAA transformant and the ΔNCU10184/NCU10184^TAA homozygous cross. (Scale bar: 20 μm.)