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. 2017 Aug 29;114(37):9948–9953. doi: 10.1073/pnas.1705338114

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Fig. 4. — Glucocorticoid induction of HIF-1 activity is independent of glucocorticoid receptor DNA binding. (A) Treatment with 10 μM DEX for 24 h activates the wild-type Tg(9xGCRE-HSV.Ul23:eGFP)^ia20 (23) glucocorticoid reporter (GRE-reporter) line, while in gr^s357 mutant background (Right) DEX is unable to activate GRE reporter. (Magnification: 14.5×.) (B) Images of wild-type and gr^s357 embryos in the Tg(4xhre-tata:eGFP)^ia21 background (HRE-reporter), treated for 24 h with DEX 10 µM. DEX activates the 4xhre-tata transgene in both wild-type and gr^s357 mutant larvae and induces a significant and generalized increase of fluorescence that is particularly evident in the liver (L in Insets). (Magnification: 14.5×; Insets, 72.5×.) (C–E) In situ hybridization of phd3 mRNA antisense probe in wild-type and gr^s357 mutants at 80 hpf. (Magnification: 17.5×.) DEX (D) or BME (E) activated phd3 gene expression in the liver (arrowheads).