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. 2017 Aug 29;114(37):9948–9953. doi: 10.1073/pnas.1705338114

Fig. S5.

Fig. S5.

Effect of GC agonist BME or DEX on epo expression. (A) BME weakly increases epo expression in zebrafish larvae. The 6-dpf larvae were exposed to 20 μM BME or vehicle (DMSO) for 8 h, and groups of 10 embryos were used to isolate RNA for qPCR (n = 8). Target-gene mRNA levels were normalized to β-actin. *P < 0.05 (t test). Graph shows mean and SEM average expression after DMSO treatment was set to 1. (B) Dexamethasone increases epo expression in the liver of adult zebrafish. Three adults were exposed to either 5 μM dexamethasone or vehicle (DMSO) for 48 h. At the end of treatment, fish were euthanized and their liver was used to isolate RNA for qPCR. The epo gene mRNA levels were normalized to acidic ribosomal protein (arp) mRNA. **P < 0.01 (t test). Graph shows mean and SEM average expression after DMSO treatment was set to 1.