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. 2017 Aug 28;114(37):9864–9869. doi: 10.1073/pnas.1705916114

Fig. S3.

Fig. S3.

Dimeric state and subunit exchange of multidomain substrates of MJ0366. (A) Subunit exchange scheme. A homodimer of GFP-17-MJ0366-ssrA created by an interaction between domains of MJ0366 exchanges its subunits with a homodimer of MJ0366-ssrA (Left) to create two heterodimers of lower molecular mass (Right). Size exclusion chromatography of (B) GFP-17-MJ0366-ssrA, (C) GFP-23-MJ0366-ssrA, (D) GFP-58-MJ0366-ssrA, (E) GFP-78-MJ0366-ssrA, and (F) GFP-117-MJ0366-ssrA followed by GFP absorbance at 500 nm. The chromatograms in green (green line) are for the multidomain substrates, in solid line for the equimolar mix of the multidomain substrates with MJ0366-ssrA, and dotted line for the mix of the multidomain substrates with an eightfold molar excess of MJ0366-ssrA. The presence of the homodimer of MJ0366 is not observed in the chromatograms since the absorbance was followed at 500 nm.