Skip to main content
. 2017 Aug 28;114(37):E7717–E7726. doi: 10.1073/pnas.1706831114

Fig. 5.

Fig. 5.

JMJD5 knockout dramatically increased the content of both arginine methylated and total H3 and H4 in mouse embryonic fibroblast (MEF) cells. JMJD7 knockout has similar effect. A and B contain two parts: (Left) Western blot of interested target and (Right) quantified results of corresponding Western blot. (A) The arginine methylation of H3 and H4 is increased in JMJD5 knockout MEFs. However, the overall content of H3 and H4 increases as well, suggesting degradation of H3 and H4 in the presence of JMJD5. (B) The knockout of JMJD7 in breast cancer cell lines leads to the increase in arginine-methylated H3 and H4, as well as the total H3 and H4. *P < 0.05 compared with WT cell, **P < 0.01 compared with WT cells.