Fig. S7.

Characterization of lymphocytes isolated from human peripheral blood. Lymphocyte subsets were purified from peripheral blood of healthy donors. Following isolation, viability, purification efficiency, and activation status were assessed by flow cytometry using Live/Dead fluorescent dye and surface antibody staining. Purity (A), activation status (B), and viability (C) of B, CD4⁺ T, and CD8⁺ T cells were assessed with anti-human CD19, CD4, CD8a, CD44/CD86, CD69/CD25 fluorophore-conjugated antibodies and Live/Dead dye (black, nonactivated cells; red, activated cells; gray, unstained cells). Purity (D) and viability (E) of B-lymphocyte subsets was assessed with anti-human CD19/CD27, IgD/IgM/IgA/IgG fluorophore-conjugated antibodies and Live/Dead dye (gray: unstained cells; orange, blue: stained cells). Data are from one donor representative of three.