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lasR quorum-sensing mutant growth in casein. (A) Growth of wild-type PA14 and a PA14 lasR mutant in M9 without NH4Cl plus 1% casein. (B) Levels of casein degradation as measured by Bradford staining throughout the course of bacterial growth. (C) Proteolytic activity of culture supernatants. Supernatants of cultures were collected after 35 h of growth. Proteolytic activity was measured using the FITC casein assay.
