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. 2017 Aug 29;114(37):E7707–E7716. doi: 10.1073/pnas.1709120114

Fig. S6.

Fig. S6.

TFG competes with Sec31A and Tango1 for Sec23 association. (A, Left) Immobilized GST-Sec31A (PRD) was incubated with recombinant Sar1B-Sec23A-Sec24A in a 1:1:2 ratio in the presence or absence of the C terminus of human TFG (amino acids 194–400), eluted using sample buffer, and separated by SDS/PAGE followed by Coomassie staining. (Right) The amount of Sec23A recovered in each case is quantified (n = 3) relative to GST-Sec31A. (B, Left) Immobilized MBP-Tango1 (amino acids 1,800–1,907) was incubated with recombinant Sar1B-Sec23A-Sec24A in the presence or absence of Sec31A (PRD) (1:1:2 ratio) or TFG (amino acids 194–400) (1:1:2 ratio), eluted using sample buffer, and separated by SDS/PAGE followed by Coomassie staining. (Right) The amount of Sec23A recovered in each case is quantified (n = 3) relative to MBP-Tango1. (C, Left) Immobilized GST-TFG (amino acids 194–400) was incubated with recombinant Sar1B-Sec23A-Sec24A in the presence or absence of Sec31A (PRD) (1:1:2 ratio), eluted using sample buffer, and separated by SDS/PAGE followed by Coomassie staining. (Right) The amount of Sec23A recovered in each case is quantified (n = 3) relative to GST-TFG. In all cases, error bars represent the mean ± SEM. **P < 0.01; *P < 0.05, calculated using a paired t test.