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. 2017 Aug 28;114(37):E7803–E7811. doi: 10.1073/pnas.1710549114

Fig. 7.

Fig. 7.

Deficient expression of HspBP1 in astrocytes contributes to high activity of CHIP in astrocytes. (A) Expression of HspBP1 in cultured astrocytes reduced the level of Ub1-CHIP, and major form of CHIP increased correspondingly (n = 3 independent experiments). (B) qRT-PCR showed that overexpression of HspBP1 did not influence transcription of CHIP in cultured astrocytes (n = 3 independent experiments). (C) HspBP1 expression in cultured astrocytes stabilized aggregated and soluble mHtt and suppressed induction of CHIP and Ub1-CHIP by mHtt, which was seen in Fig. 1D (n = 3 independent experiments). (D) Induction of Hsp70, CHIP, and Ub1-CHIP by heat-shock stress at 42 °C for 1 h was significantly inhibited by HspBP1 expression in DIV 30–50 astrocytes (n = 4 independent experiments). (E) Expression of HspBP1 decreased basal levels of Hsp70 in astrocytes (n = 3 independent experiments). *P < 0.05, **P < 0.01, ****P < 0.0001 (unpaired two-tailed Student’s t test). Data are represented as mean ± SEM (error bar).