Table 1.

PCR primers for generation of anti-HER2 and anti–CSPG4 Fc mutant antibodies.

Primer name	Sequence 5′→3′	Mutation	Amplified fragment
F-DE-1	CAGCACCTGAACTCCTGGGGGGACCGGTCTTCCTCTTCCCCC	S239D	DE F1
R-DE-1	GGGGCTGCCCTTTGGCTTTGGAGATGGTTTTCTCGGGGGCTGG	I332E	DE F1
F-DE-2	CCAGCCCCCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCCC	I332E	DE F2
R-pVitro-1-Kappa	ACCGCGGCTAGCTGGAACCCAGAGCAGCAGAAACCCAATG	–	DE F2
F-pVitro-VL-Univ	CATTGGGTTTCTGCTGCTCTGGGTTCCAGCTAGCCGCGGT	–	DE F3
R-DE-3/R-NQ-3	GGGGGAAGAGGAAGACGTCCGGTCCCCCCAGGAGTTCAGGTGCTG	–	DE F3; NQ F3
F-DE-4/F-NQ-4	GTTGCTTTGATTACAACACTGGAGAGAAATGCAGCATGTTGCTGATT	–-	DE F4; NQ F4
R-DE-4	GGGGGAAGAGGAAGACCGGTCCCCCCAGGAGTTCAGGTGCTG	S239D	DE F4
F-NQ-1	AGCACGTACCGGGTGGTCAGCGTCCTCACCGTCCTGCACCAG	N297Q	NQ F1
R-NQ-1	TGATCTACCCGCGCTCAGCCCTGGGCGCATGCTCCTCGCGCTGTC	–	NQ F1
F-NQ-2	CGAGGAGCATGCGCCCAGGGCTGAGCGCGGGTAGATCAGAGCACA	–	NQ F2
R-NQ-2	TACAAAGTGTTACCCCTCTAGACCTGGAAAGACCAGGCGGAGTTT	–	NQ F2
F-NQ-3	GCCTGGTCTTTCCAGGTCTAGAGGGGTAACACTTTGTACTGCGTT	–	NQ F3
R-NQ-4	AGGACGGTGAGGACGCTGACCACCCGGTACGTGCTGTACTGC	N297Q	NQ F4

Underlined nucleotides depict mutated codons; sequences in red font indicate mutated nucleotides.