Table 3.

Characteristics of the Expi293F vs. the HEK293-F cloning/expression platforms.

Expression host	Expi293F	HEK293-F
Wild-type (WT) antibody polymerase incomplete primer extension (PIPE) cloning	Yes	Yes
Generation of Fc mutants	Yes	No
Expression culture conditions	Serum free	10% FBS
Expression system type	Transient	Stable
Antibody concentration (sup)	130 µg/mL (anti-CSPG4 WT)	13 µg/mL (anti-CSPG4 WT)
Working culture volume (expression)	30 mL	500 mL
Time required for cloning/sequencing verification	3 days	3 days
Time required for large scale plasmid DNA production/transfection	2 days	2 days
Time required for expression/purification	1 week	4 weeks
Total time required (whole platform)	12 days	33 days