Figure 2.

Developmental capacities of two-cell embryos isolated from control and obese mice and cultured for 72 h in vitro. The graph shows the proportion (%) of arrested embryos (two-cell stage and degenerates) and embryos that reached higher developmental stages (3- to 16-cell stage, morulas and blastocysts). Embryos from control mice were cultured in the leptin-free media (n = 105) or media supplemented with leptin at 10 ng/mL (n = 134). Embryos from obese mice were cultured in the leptin-free media (n = 101) or media supplemented with leptin at 10 ng/mL (n = 98). Asterisks indicate statistical differences: chi-square test with three degrees of freedom, *P < 0.05; ***P < 0.001.