Table 4.
Qualitative parameters of in vitro-obtained blastocysts originating from control and obese mice.
| Control mice |
Obese mice |
|||
|---|---|---|---|---|
| 0 ng/mL leptin | 10 ng/mL leptin | 0 ng/mL leptin | 10 ng/mL leptin | |
| Number of evaluated blastocysts (n) | 72 | 63 | 60 | 69 |
| Mean number of cells per blastocyst | 67.78 ± 2.06 | 71.13 ± 2.33 | 63.65 ± 2.06 | 67.30 ± 2.28 |
| Cell differentiation (% ICM:TE cells) | 34.37:65.63 | 29.27:70.73 | 34.63:65.36 | 34.60:65.40 |
| ICM/TE ratio | 0.56 ± 0.03 | 0.44 ± 0.04 | 0.58 ± 0.06 | 0.59 ± 0.05 |
| Average % of dead cells in blastocysts | 6.23 ± 0.54a | 5.89 ± 0.59a,b | 6.14 ± 0.54a | 4.15 ± 0.35b |
| Distribution of dead cells (% in ICM:TE) | 88.46:11.54 | 84.91:15.09 | 99.11:0.89a | 93.92:6.08a |
| Average % of dead cells in ICM line | 18.43 ± 1.90a | 18.22 ± 1.86a | 21.71 ± 3.62a | 11.74 ± 1.15b |
| Average % of dead cells in TE line | 1.51 ± 0.43a | 1.37 ± 0.29a | 0.14 ± 0.09b | 0.50 ± 0.15b |
The results are expressed as the mean values ± SEM. Different letters in superscript indicate statistical differences; mean number of cells: ANOVA followed by Tukey’s test; ICM/TE ratio, average % of dead cells in blastocysts, in ICM line, and in TE line: Kruskal–Wallis followed by Dunn’s test; the distribution of dead cells between ICM and TE lines: chi-square test.