Figure 1.

Assembled 3D skin explant model for anaerobic bacterial infection. (A) Timeline of the model infection highlighting the time points where biopsies were placed into RNAlater or 10% NBF. (#) Time points where the culture media were collected for cytokine measurement. Culture media without antibiotics was used between −4 and 4 h. Culture media containing antibiotics was used between 4 and 28 h after bacterial exposure. (B) Schematic overview of the assembled model at 0–28 h (exposure to Dichelobacter nodosus). (C) Photo of the assembled model in a 12 well-plate.