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Schematic representation of crossing scheme involved in the generation of chromosome specific translocation line [1EL (1AS)]. Double monosomics were created by carefully selecting the breeding material and screening was carried out by combination of protein markers and genomic in situ hybridization. Multiple rounds of backcrossing were carried out to recover the background of recipient cultivar. N1AT1D = Genetic stock nullisomic for chromosome 1A and tetrasomic for chromosome 1D.
