Table 3.
Effect of different carbons on degradation of 2C5NP by strain CNP-8.
| Carbon source | Concentration (g/L) | OD600a | Time required for complete degradation of 2C5NP (h) | Degradation rate (μM h−1)b |
|---|---|---|---|---|
| Control | - | 0.171 ± 0.012 | 42.4 ± 2.5 | 9.43 |
| glucose | 0.5 | 0.332 ± 0.026 | 24.7 ± 1.6 | 16.19 |
| 5 | 1.587 ± 0.035 | 60.1 ± 2.4 | 6.65 | |
| succinate | 0.5 | 0.354 ± 0.018 | 22.5 ± 2.1 | 17.78 |
| 5 | 1.632 ± 0.027 | 53.3 ± 3.8 | 7.50 | |
| lactate | 0.5 | 0.312 ± 0.032 | 26.5 ± 1.4 | 15.09 |
| 5 | 1.534 ± 0.024 | 63.4 ± 3.5 | 6.31 |
Minimal salt medium (MSM) with 0.4 mM of 2C5NP was inoculated by strain CNP-8 with initial OD600 of 0.05, and shaken at 30°C and 180 rpm. Control: without added carbon source.
a
OD600 was determined at the time of complete degradation of 2C5NP.
b
Rate of 2C5NP degradation (μM h−1) = 400/time required for complete degradation of 2C5NP.